Explore cutting-edge genomics technologies and their applications in elucidating principles of gene regulation in mammalian cells through this 54-minute seminar by Bas van Steensel from the Netherlands Cancer Institute. Delve into an innovative approach for efficiently altering the linear structure of large genomic loci in a highly multiplexed manner, and examine its impact on gene regulation and spatial chromatin organization. Learn about enhancer-promoter interactions, the effects of gene and regulatory element locations on function, and scalable DNA element manipulation techniques. Investigate the formation of strong barriers for activation by specific control regions and the role of loop extrusion in gene regulation. Gain insights into lamina-associated domains (LADs) and explore more scalable strategies for understanding genome organization and function.