Explore the fascinating world of brain mosaicism and its implications for neurodevelopmental disorders in this 25-minute webinar presented by Dr. Tracy Bedrosian. Delve into the cutting-edge research that combines high-throughput single-cell 3'-RNA-sequencing with targeted Iso-Seq long-read RNA sequencing to uncover how somatic mutations contribute to developmental brain malformations. Learn about the challenges in determining the impact of mosaic mutations on cell lineages and individual cell function, and discover how this innovative approach is applied to surgically-resected patient brain tissue. Gain insights into the types of somatic mutations, malformations of cortical development, and the process of identifying mosaicism in patients. Examine a case study on hemimegalencephaly, focusing on somatic variants in PTEN and their predicted protein effects. Understand the integration of genotype and cell type data, and explore the clinical implications of this research. This webinar offers a comprehensive overview of the latest advancements in deciphering brain mosaicism using long-read sequencing techniques, providing valuable knowledge for researchers and clinicians in the field of neurodevelopmental disorders. Read more