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Intro
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Count Rumford one of the founding members of the Royal Institution
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J.J. Thomson unveiled the discovery of the electron
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First mass spectrometers
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Mass spectrum of aspirin
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Mass Spectrum of Melanocyte Stimulating Hormone
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Nobel prize for chemistry 2002
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Electrospray - without unfolding
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Folding in the presence of molecular chaperones
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A new phase for structural biology?
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Proteins with many different conformations
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The passage of complexes through the mass spectrometer
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Scientific goals and questions
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Unraveling polydispersity - the alpha crystallins
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Disassembly predicts evolutionary assembly pathways
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Mass spectrometry to identify the building blocks within complexes
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Micelles protect membrane complexes and lipid binding remains intact
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From micelles to membrane proteins in the gas phase
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ATP Synthase - 95:1 DDM: protein
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Mass spectrum of intact ATPase from Thermus thermophilus
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Mass spectral assignment strategy - Massign
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New ways to study how small molecules stabilise membrane proteins
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Monitoring unfolding of AqpZ as a function of POPG binding
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P-glycoprotein - an ABC transporter and drug efflux pump
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Looking for a small difference
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Mechanisms of drug resistance
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Zinc binding to a human metalloprotease ZMPSTE24
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Progeria is a common side effect of anti- HIV drugs
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Processing of lamin A peptides is inhibited by drug binding
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Sugar transporter semiSWEET - remodeling the lipid bilayer
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From Neon to rotary motors to flipping lipids and drugs
Description:
Explore a groundbreaking lecture on structural biology that revolutionized the field by studying proteins in the vapor phase. Delve into Carol Robinson's innovative research, which provides a new perspective on protein interactions and their behavior beyond solid and solution phases. Learn how this unconventional approach has proven invaluable in understanding dynamic interactions between proteins, lipids, and drugs. Discover the journey from early mass spectrometry techniques to cutting-edge applications in studying membrane proteins, drug resistance mechanisms, and the impact on human health. Gain insights into the scientific goals, methodologies, and breakthroughs that have shaped this new phase of structural biology, including the analysis of complex protein structures, evolutionary assembly pathways, and the role of lipids in protein stability.

A New Phase for Structural Biology - With Carol Robinson

The Royal Institution
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