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Study mode:
on
1
Acknowledgements
2
Cellular compartments
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Outline
4
Membraneless compartments
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granules
6
granule assembly gradient
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granules are liquid drops
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Liquid-liquid phase separation
9
Phase transition in a cell
10
Phase diagram
11
Active processes: fluctuations
12
Thermodynamics of phase coexistence
13
Droplet coexistence
14
In vitro droplet ripening
15
Ostwald ripening
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Droplet fusion: hydrodynamics
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Cell polarity
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Protein gradient drives granule segregation
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RNA binding competition
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Stochastic droplet dynamics
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Concentration buffering
22
Stochastic protein production
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Noise buffering by phase separation
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Noise buffering in Experiments
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Condensates as chemical reaction centers
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Droplet turnover: detailed balance
27
Chemically active droplets
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Steady state of active droplets
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Dynamics of active droplets
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RNA-protein assemblies organize chemistry in space
31
Droplets in early life?
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Active droplets as simple models for photocells
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Division of active droplets
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Growth-division cycles
35
Hardening of protein condensates
36
Pulling on condensates: material properties
37
Surface tension from active micro-rheology
38
Time periodic forcing
39
Aging of protein condensates
40
Increasing relaxation time: glassy dynamics
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Gel formation versus aging glass
42
Glassy dynamics: disorder of
43
Conclusions
Description:
Explore the fascinating world of phase separation in living cells through this comprehensive 1 hour 25 minute lecture by Frank Jülicher from the International Centre for Theoretical Sciences. Delve into topics such as membraneless compartments, liquid-liquid phase separation, droplet dynamics, and protein condensates. Discover how cells utilize noise and fluctuations to drive essential processes. Examine the thermodynamics of phase coexistence, stochastic protein production, and the role of condensates as chemical reaction centers. Investigate active droplets as models for early life and photocells, and learn about the aging and material properties of protein condensates. Gain insights into the intersection of statistical physics and biology in this interdisciplinary exploration of cellular phenomena.

Phase Separation in Living Cells by Frank Jülicher

International Centre for Theoretical Sciences
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