Explore the relationship between neutral hydrogen gas in galaxies and the cosmic web in this 27-minute conference talk from the "Co-evolution of the Cosmic Web and Galaxies across Cosmic Time" conference. Delve into the MIGHTEE-HI survey, examining how the cosmic web influences galaxy formation and evolution. Discover the observational techniques, data sources, and parameters used to study HI alignment and distribution. Learn about the limitations of current radio telescopes and the challenges in detecting the spin transition of neutral hydrogen. Gain insights into the barrier mass concept and its implications for galaxy formation. Conclude with key takeaways and potential future research directions in this fascinating field of astrophysics.