Save Big on Coursera Plus. 7,000+ courses at $160 off. Limited Time Only! Grab it Explore the intricacies of RT-qPCR in gene expression analysis through this 45-minute webinar presented by Jennifer Sandoval, M.S., a Technical Scientist at Thermo Fisher Scientific. Delve into the critical aspects of the RT-qPCR workflow, including RNA isolation and reverse transcription, and their impact on accurate gene expression quantitation. Learn about reverse transcriptase attributes, such as RNase H activity, thermostability, processivity, sensitivity, and inhibitor tolerance. Discover strategies for working with challenging RNA samples, including low-input RNA, limited sample sources, and samples with high structural complexity. Address common issues like genomic DNA contamination and explore solutions for various sample types, including archived tissue, blood, and plant samples. Gain valuable insights to optimize your RT-qPCR experiments and push the boundaries of gene expression analysis.