Learn how to create a Region of Interest (ROI) in OpenCV using mouse events and clicks. Explore techniques for defining window parameters, setting up mouse event functions, and implementing a while loop to capture and process frames. Master the process of copying the ROI and troubleshoot common issues. Gain hands-on experience with practical examples and step-by-step guidance for running Python files effectively in this comprehensive video lesson on AI development for the Jetson Nano platform.