Introduction to Genomics

Introduction to Genomics

Dive into a comprehensive 18-hour course on genomics, covering fundamental concepts and advanced techniques in molecular biology and genetics. Begin with a review of genome content, gene structure, transcriptional regulation, and repetitive sequences. Explore various DNA sequencing methods, including Sanger, shotgun, Illumina SBS, PacBio, and Nanopore technologies. Learn about genome assembly techniques, forward and reverse genetics, and gene editing methods, with a focus on CRISPR-Cas9 technology and its applications. Investigate sequence alignments, transcription factor-DNA interactions, and epigenomics, including histone modifications, DNA methylation, and chromatin accessibility. Delve into RNA sequencing techniques, both bulk and single-cell, and their analysis methods. Examine the physical organization of the genome, chromatin conformation capture assays, and molecular interaction networks. Conclude with an exploration of human genetic variation, genome-wide association studies (GWAS), and their implications for understanding complex traits and diseases. Read more