Automatic Cell Annotation for Single-Cell RNA-Seq Data - Multiple Reference Strategies in SingleR - Part 2
Explore advanced techniques for cell type annotation in single-cell RNA-Seq data using multiple reference datasets in this comprehensive video tutorial. Learn various strategies for annotation, including using combined references, comparing scores across references, and employing harmonized labels. Discover how to fetch reference datasets from the celldex package, visualize results in UMAP plots, and create diagnostic heatmaps. Gain insights into the strengths and pitfalls of each approach, understand how to handle label inconsistencies across references, and learn to map cell ontology terms to labels. Follow along with practical demonstrations using SingleR and delve into the intricacies of cell type annotation to enhance your bioinformatics skills.