How to Analyze Single-Cell RNA-Seq Data in R - Detailed Seurat Workflow Tutorial

YouTube·Bioinformatics

Dive into a comprehensive tutorial on analyzing single-cell RNA sequencing data using R and the Seurat package. Follow a detailed workflow for processing a 10X Genomics dataset, covering essential steps from data download to UMAP visualization. Learn to read count matrices, create Seurat objects, perform quality control, normalize data, find variable features, scale data, and understand different data slots. Explore dimensionality reduction techniques, including PCA and UMAP, and delve into clustering methods with a focus on resolution. Gain practical insights into bioinformatics analysis for genomics research, suitable for beginners and experienced researchers alike.