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1
Intro
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Human Tissue Models for better therapies
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Meet the OrganoPlate
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OrganoPlate® family
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Immune cell migration
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Need of novel in vitro immune migration models
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Based on publication
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Formation of HMEC-1 vessels in the OrganoPlate 3-lane
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HMEC-1 vessels can be maintained in immune cell medium
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Visualization and quantification of T cells in microfluidic chip
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Perfusion of HMEC-1 vessels with fluorescently labelled T cells
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ET cells and vasculature interplay
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Generation of chemokine gradients in the OrganoPlate 3-lane
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T cell extravasation in the presence of chemokine gradients
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Donor-to-donor differences are preserved
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Induction of vascular inflammation Upregulation of adhesion molecules
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Blocking extravasation of T cells
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Immune infiltration in the tumor compartment
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Immune migration assays are also compatible with HUVECS
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The OrganoPlate as a tool to study immune cell extravasation and infiltration
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Cell migration in the OrganoPlate
Description:
Explore cell migration mechanisms in a 37-minute webinar featuring Dr. Kristin Bircsak's presentation on 3D cell migration using a microfluidic endothelium-on-a-chip model. Delve into the essential process of cell migration and its role in wound healing, cancer metastasis, and immune system function. Learn about the OrganoPlate technology and its applications in studying immune cell extravasation and infiltration. Discover how to visualize and quantify T cell migration, generate chemokine gradients, and induce vascular inflammation in microfluidic chips. Gain insights into donor-to-donor differences, blocking T cell extravasation, and the compatibility of immune migration assays with various cell types. Understand the potential of human tissue models for developing better therapies and advancing our understanding of complex biological phenomena.

3D Cell Migration Using a Microfluidic Endothelium-on-a-Chip Model

Labroots
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