A Saliva-Based RT-PCR SARS-CoV-2 Detection Assay - Development and Deployment
Explore a 30-minute webinar presented by Suzie Alarcon, CGMBS, MB(ASCP)ᶜᴹ, focusing on the development and deployment of a saliva-based RT-PCR SARS-CoV-2 detection assay. Learn about a One-Step RT-qPCR assay based on the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel, designed to detect SARS-CoV-2 particles from heat-inactivated and directly lysed saliva samples. Discover how this method eliminates the need for RNA extraction and utilizes human RNAseP detection as an internal control in a multiplexed reaction. Gain insights into the assay's validation process using positive clinical samples and its high sensitivity of 94.7% or higher in 6μl reactions. Earn PACE credits by watching the webinar and following the provided instructions.