Learn to perform scratch assay analysis efficiently using Python in this 28-minute video tutorial. Discover how to segment time series images for 'clean' versus cell-filled regions in wound healing assays, a common biological technique. Explore advanced segmentation methods using Entropy filter and Otsu thresholding to overcome the limitations of histogram segmentation. Master the process of looping through images, analyzing data, and plotting results with just five lines of code. Gain insights into entropy filters, image processing, binary image creation, and linear regression. Access the complete code on GitHub to enhance your microscopy image analysis skills.