Confocal Microscopy and Its Applications in Biomolecular Diffusion Studies - Lecture 1
Save Big on Coursera Plus. 7,000+ courses at $160 off. Limited Time Only! Grab it Explore confocal microscopy and its applications in a 32-minute video presentation by Fatma Pri-Cakmak from MIT. Delve into the history of microscopes, modern microscopy techniques, and laser scanning confocal microscopy. Learn about the diffusion of biomolecules in vesicle-coated RNA-based coacervates and how cellular activities can be compartmentalized by phase separation. Discover the PolyU/spermine coacervate system, partitioning in coacervates, and concentration calculations using microscopy. Understand Fluorescence Recovery After Photobleach (FRAP) techniques for studying partial and entire droplet diffusion. Examine vesicle assembly around coacervate droplets and RNA diffusion in these structures. Part of the NACK - Nano-Educators Topical Seminar Series, this comprehensive overview provides valuable insights into advanced microscopy techniques and their applications in studying complex biological systems.