Tips and Tricks for Successful Multiplexing IHC
Save Big on Coursera Plus. 7,000+ courses at $160 off. Limited Time Only! Grab it Discover essential techniques for successful multiplexing immunohistochemistry (IHC) in this informative webinar presented by neuroscientist and microscopist Darpan Saraswat. Learn how to optimize sample preparation, prevent autofluorescence, and minimize unspecific antibody labeling in complex samples like tumor microenvironments. Explore strategies for selecting appropriate markers, fluorophore dyes, and labeling techniques to expand your IHC experiments. Gain insights into avoiding fluorescent bleed-through and spectral overlap, and understand the importance of controls in multiplexed IHC. Master the art of planning and executing larger Spatial Imaging panels while considering factors such as fluorophore photobleaching and photostability. Enhance your skills in advanced microscopy methods and high-resolution imaging techniques to elevate your research in cellular analysis.