One-Step qPCR Master Mix for Amplification from Crude or Purified Samples
Explore a 25-minute video on qPCR reagent research tools and the PrimeTime™ One-Step 4X Broad-Range qPCR Master Mix from Integrated DNA Technologies (IDT). Discover how this versatile master mix enables amplification from both extracted and crude samples. Gain insights into using IDT primers, probes, and master mix products for SARS-CoV-2 tracking via qPCR. Learn about the advantages of the PrimeTime™ 1-Step 4X Broad-Range Master Mix, including its building blocks, high endpoint fluorescence, and excellent multiplexed limit of detection with purified RNA templates. Explore its superior inhibitor resistance against heparin, hematin, and humic acid, as well as its extraction-less direct amplification capabilities. Compare the performance of IDT's master mix in amplifying viral analytes from extracted and non-extracted human samples, and see how it outperforms competitors in terms of lower Ct values and higher endpoint fluorescence when using human saliva and nasopharyngeal matrix.