Post-Genome Assembly
Dive into an extensive 8-hour bioinformatics tutorial covering post-genome assembly techniques for bacterial genomes. Learn genome annotation using PROKKA, visualization with Artemis and DNAPlotter, and assembly and annotation using PGAP. Explore identification of insertion sequence elements using ISfinder and genome visualization with CGView. Gain hands-on experience in pangenome analysis, antimicrobial resistance gene identification with Abricate, and virulence factor detection. Master phylogenetic tree construction using RAxML, iTOL, MUSCLE, and PhyML. Delve into variant calling techniques with BCFTOOLS and Snippy. Conclude with comparative genomics and genome comparison using BRIG software, equipping yourself with essential skills for bacterial genome analysis.