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1
Intro
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Session Objectives
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Illumina Sequencing Workflow
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Library Prep is Critical for Successful Sequencing
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What is a Cluster?
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What is a flow cell?
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Flow cell Architecture Random vs Pattemed
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Patterned flow cells Complete control of pitch & feature size
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Hybridize Fragment & Extend
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Denature Double-Stranded DNA
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Single-Stranded DNA
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Bridge Amplification
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Denature Double-Stranded Bridge
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Linearization
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Reverse Strand Cleavage
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Read 1 Primer Hybridization
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Patterned Flow Cell and ExAmp Technology
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Illumina Sequencing Systems
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Dye Chemistry 4-channel chemistry
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2-Channel SBS Chemistry
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Dye Chemistry 2-channel chemistry
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Sequencing by synthesis with CMOS detection 1-channel chemistry
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Illumina Chemistry Comparison
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Paired-End Sequencing
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Single Index Reads
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Dual Index Reads
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Analysis Overview
Description:
Explore an in-depth webinar on Illumina sequencing, covering the entire process from library preparation to data analysis. Gain insights into the Illumina Sequencing Workflow for Next Generation Sequencing, including library preparation constructs, cluster generation, and sequencing by synthesis. Learn about the biochemical processes automatically performed by Illumina sequencing devices and compare different chemistry versions and instrument engineering developments. Presented by Scott Westenberger, PhD, Staff Clinical Field Applications Scientist at Illumina, this 52-minute session provides a comprehensive overview applicable to various assays across different sequencing instruments. Delve into topics such as flow cell architecture, bridge amplification, dye chemistry, paired-end sequencing, and analysis overview. Moderated by Tara Namey, MS, LCGC, Senior Genomic Science Liaison at Ambry Genetics, this webinar offers valuable knowledge for professionals in molecular biology, genetics research, and clinical oncology. Read more

Illumina Sequencing Overview - Library Prep to Data Analysis

Ambry Genetics
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