Explore the latest advancements in single-cell RNA sequencing technology in this 44-minute lecture from the Medical and Population Genetics Primer series at the Broad Institute. Delve into the Ultima sequencing platform's mostly natural sequencing-by-synthesis approach for scRNA-seq. Learn about experimental design, read quality assessment, downsampling techniques, and quality control measures. Discover how to analyze biological data, including donor labels and individual genes, using tools like Azimuth and Mimoska. Gain insights into single-cell screening methods, non-significant gene interpretation, and alternative data types. The lecture concludes with a discussion on mitochondrial analysis and a Q&A session, providing a comprehensive overview of cutting-edge scRNA-seq methodologies for researchers and students in the field of genetics and genomics.