Explore a comprehensive lecture on the intersection of synthetic biology, machine learning, and automation in biomanufacturing. Delve into the development of biofoundries, genome-scale engineering tools, and the integration of biocatalysis and chemical catalysis for advanced synthesis. Learn about the Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB), genome-wide CRISPR systems, and the potential of AI-enabled synthesis planning. Discover how these technologies are revolutionizing the production of chemicals, materials, and therapeutic agents. Gain insights into the challenges and opportunities in this rapidly evolving field, including the newly launched NSF AI research institute for molecular synthesis. Examine case studies on automated protein engineering, preventing mitochondrial disease transmission, and enzymatic reduction of olefins. Understand the vision for developing an Automated Cellular Engineering (ACE) platform and the future of biomanufacturing.