Explore high-throughput, high-performance algorithms for high-resolution subtomogram averaging in this 47-minute conference talk by Mikhail Kudryashev from the Stiftung Max-Delbrück-Centrum für Molekulare Medizin. Delve into the powerful method of subtomogram averaging from cryo-electron tomograms for determining macromolecular structures in their native state. Discover the tools developed for in situ structural biology, including TomoBEAR for near-automated tomographic processing and SUSAN for high-performance computing. Learn about applications to protein lattices, coats, ribosomes, and membrane proteins, with a focus on overcoming throughput and processing time bottlenecks. Examine the structure of an ion channel determined at side-chain resolution using these advanced tools. Gain insights into sample purification, excitation-contraction coupling in skeletal muscle, and the structure of RyR1 in native membranes. Compare reported structures of RyR1 and explore optimization techniques for subtomogram averaging, including CTF estimation and 3D reconstruction modules in SUSAN. Read more