Explore the intricacies of cargo transport regulation in living organisms through this comprehensive lecture. Delve into the essential roles of kinesin and dynein in neuronal function and viral propagation. Learn about advanced experimental techniques like optical traps for measuring motor motility and force production. Examine various types of transport regulation, including global and semi-global regulation of kinesin-family motors. Investigate the adaptation properties of cargoes and their ability to escape optical traps. Discover the importance of nuclear migration and the roles of specific proteins like NudE and Lis1. Gain insights into mitochondrial interactions, lipid droplet movement, and their significance in energy production and bacterial defense. Conclude with an overview of open questions in lipid droplet transport and the researchers behind this work.