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1
Intro
2
Electromagnetic spectrum
3
What happens? Example: ketone
4
Molecular spectroscopy
5
Principles of spectroscopy
6
Principles of fluorescence
7
Tryptophan fluorescence
8
Fluorescence spectroscopy
9
Internal relaxation
10
Fluorescence dictionary - Part 11
11
Varian Eclipse
12
Xenon flash lamp
13
Instrumentation - PMT detector
14
Fluorophores - Molecular structure
15
Flourophores
16
Factors affecting the fluorescence signal
17
Concentration - Ideal conditions
18
Inner filter effect
19
Problem with the correction
20
Environment - Solvent
21
Environment - Temperature
22
Environment - Denaturant
23
Dynamic quenching
24
Static quenching
25
Non-radiative energy transfer
26
Scatter
27
Ways to measure fluorescence - Polarization
28
Ways to measure fluorescence - Time-decay
29
Fluorescence summary
30
Why fluorescence?
31
Options of measuring fluorescence
32
Second Order Advantage - PLS VS. PARAFAC
33
Proteins and salt solutions
Description:
Explore the principles and applications of fluorescence spectroscopy in this comprehensive 51-minute webinar presented by Aasmund Rinnan. Delve into the electromagnetic spectrum, molecular spectroscopy, and the fundamental principles of fluorescence. Learn about fluorophores, factors affecting fluorescence signals, and various measurement techniques including polarization and time-decay. Discover the advantages and limitations of this highly sensitive method capable of measuring compounds at ppb levels. Examine practical examples such as tryptophan fluorescence and the effects of environment, concentration, and quenching on fluorescence signals. Gain insights into advanced topics like the Second Order Advantage and the comparison between PLS and PARAFAC methods. Understand the applications of fluorescence spectroscopy in analyzing proteins and salt solutions, equipping yourself with valuable knowledge for research and analytical purposes.

Fluorescence in One Hour

Chemometrics & Machine Learning in Copenhagen
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