Explore the application of molecular methods in diagnostic microbiology through this informative lecture by Dr Alice Wort. Gain practical insights from her experience as a Microbiologist at the largest NHS laboratory in Northeast England, specializing in high-throughput molecular screening. Discover how state-of-the-art technology and clinical expertise are utilized to enhance patient care. Learn about various molecular techniques, including real-time PCR, high-throughput qualitative and quantitative methods, batch qualitative analysis, rapid PCR, and 16S PCR. Understand the challenges and future prospects in the field, including true point-of-care testing. Delve into topics such as SARS-CoV-2 testing, serology, antigen/toxin detection, and proteomics using MALDI-TOF. This comprehensive overview provides valuable knowledge for professionals and students interested in the latest advancements in diagnostic microbiology.