Learn about the bacteriological diagnosis of tuberculosis through smear microscopy and culture techniques in this comprehensive 30-minute video. Explore conventional diagnostic methods, including sputum collection and triple packaging of infectious material. Examine the appearance of TB bacilli in smears and understand the limitations of smear microscopy. Discover advancements like Light Emitting Diode (iLED) microscopy and solid culture techniques using Lowenstein Jensen and agar media. Delve into liquid culture systems, focusing on the MGIT960 method, its components, principles, and result interpretation. Investigate species identification of non-tuberculous mycobacteria through biochemical tests and molecular techniques. Gain insights into whole genome sequencing for NTM and grasp key messages for effective TB diagnosis.