Explore a comprehensive lecture on early cancer detection using plasma cell-free DNA (cfDNA) and targeted methylation assays. Delve into the challenges of single cancer screening and the potential of multi-cancer early detection tests. Learn about the biology of methylation patterns in cancer detection, clinical development programs, and large-scale studies like the Circulating Cell-free Genome Atlas (CCGA). Discover the key performance features of multi-cancer early detection tests and their potential integration into existing clinical workflows. Gain insights into the PATHFINDER study, which assesses the implementation of these tests in real-world clinical settings.