Explore the fundamentals of cryo-electron microscopy and single-particle analysis (SPA) in this comprehensive webinar presented by Prof. Michael Unser from EPFL. Delve into the high-resolution imaging of biosamples in their native state, understanding the X-ray transform model for electron ray transmission, and the challenges of tomographic reconstruction in SPA. Learn about current reconstruction paradigms, regularized reconstruction schemes, and the innovative CryoGAN algorithm. Examine synthetic experiments, discriminator architecture, and results in structural reconstruction. Address the key challenge of structural heterogeneity, exploring methods for reconstructing both continuous and discrete sets of conformations. Gain valuable insights into advanced signal processing techniques applied to cutting-edge biological imaging.