Explore a comprehensive lecture on developing computational models of the brain and advanced tools for mapping and manipulating neural circuits. Delve into the structure of neurons, action potentials, and the unknowns in neuroscience. Learn about innovative techniques such as polymer mesh expansion, superresolution microscopy, and lightsheet microscopy for scalable brain imaging. Discover protocols for expansion microscopy and its applications in visualizing microtubules and synapses. Gain insights into cutting-edge approaches for quantitative analysis of neural structures, including the fruit fly brain and Brainbow technology. Understand the potential impact of these tools on unraveling the complexities of brain function and advancing our understanding of neuroscience.