Learn to denoise 2D and 3D multichannel scientific images using the Noise2Void deep learning approach in this 22-minute tutorial. Explore the process of denoising CZI images from ZEISS light microscopes using the czifile library, with techniques applicable to various scientific image formats. Discover how Noise2Void learns directly from noisy images without requiring clean data, making it ideal for confocal image denoising. Understand the underlying assumption that signal has structure while noise does not, enabling signal prediction from surrounding pixels. Access provided code examples for both 2D and 3D multichannel denoising, and gain insights into data generation, model training, and plotting. Suitable for researchers and microscopists working with complex scientific imagery.