Explore a conference talk from AbGradCon 2023 focusing on microbial metabolism in acidic brines of Western Australia. Delve into Emily Paris's research on water activity, metabolic activity, and the limits of life in extreme environments. Learn about single-cell analysis techniques, nano SIM results, and the implications for Mars analogs. Gain insights into the relationship between water activity and pH, and examine data on the percentage of active cells in these harsh conditions. Understand the significance of this research for astrobiology and the search for life in extreme environments on Earth and potentially on other planets.