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Intro
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Significance of Foodborne Pathogens in the US
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Unknown or Unspecified Agents
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Detection Approach: Does One Method Fit All? Ready-to-eat products: 97-99.9% are negative Raw uncooked products: 50-99% are negative
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Biosensor-Based Detection
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Forth Coming Technologies for High Throughput Screening
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Online Inspection
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Pathogen Detection: Nanobiosensor Approach Sample
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Fiber Optic Sensor for Specific Pathogen Detection Advances Capture molecule: Antibody, Receptor Antibody, Aptamer
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Antibody-Aptamer fiber Optic Sensor for L. monocytogenes
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Immunomagnetic Separation and Fiber- Optic Sensor
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Fiber Optic Sensor for Listeria monocytogenes
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Summary of Fiber Optic Sensor Results for Pathogens Pathogen Detection Detec. Publications
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Multi-Pathogen Detection Strategy
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Functional Biosensing: a Modern Approach
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Mammalian Cell-Based Sensor
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Cell-Based Sensor (3D) for High Throughput Screening
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Visual Screening for Toxins
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Light scattering sensor (BARDOT)
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Evolution of BARDOT
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Scatter patterns of Listeria species on BHI
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BARDOT Analysis of Magnetic Bead Captured Listeria
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Salmonella Detection
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Comparison of Scatter Patterns
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Detection of Top-20 Salmonella Serovars
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Salmonella Enteritidis Detection from Raw Chicken
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Shiga-toxin Producing E. coli Detection
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Detection of E. coli O157:H7 from Ground Beef
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Detection of STEC (026, 0157) from Food
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Detection of Vibrio from Oyster
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Bacillus Detection from Raw Milk
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Natural Microbial Community Analysis
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Cantaloupe Microbial Diversity before and after atmospheric cold
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Summary and Final Thoughts
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Funding: USDA, NSF, NIH and Center for Food Safety Engineering
Description:
Explore novel biosensor technologies for high-throughput screening of pathogens in this 43-minute lecture by Professor Arun K Bhunia from Purdue University. Delve into the significance of foodborne pathogens in the US and learn about cutting-edge detection approaches, including fiber optic sensors, immunomagnetic separation, and light scattering technology (BARDOT). Discover multi-pathogen detection strategies, functional biosensing using mammalian cell-based sensors, and visual screening for toxins. Examine case studies on detecting various pathogens like Listeria, Salmonella, E. coli, and Vibrio in different food products. Gain insights into natural microbial community analysis and the latest advancements in pathogen detection for food safety.

Novel Biosensor Technologies for High Throughput Screening of Pathogens

American Society for Microbiology
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