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Intro
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Soluble proteins are actively imported into the nucleus, through binding of the NLS
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How do proteins traffic to the Inner Nuclear Membrane?
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Models for membrane protein transport to the INM
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Transport-Factor Mediated Trafficking of inner nuclear membrane proteins
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Experimental Questions
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Targeting a tail anchored ER protein to the nuclear envelope
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Measuring NE enrichment using nuclear localization index NLI
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Addition of an NLS to an chimeric membrane protein results in NE enrichment
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Defining Rules for NE Targeting
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NLS fused chimeric membrane proteins access
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Working model of NLS-mediated INM protein trafficking
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Addition of a monopartite NLS is sufficient to enrich an artificial membrane protein at the NE
Description:
Explore a detailed InDepth session from the SEB Annual Conference 2021 focusing on the targeting of tail-anchored membrane proteins to the inner nuclear envelope in plants. Delve into Norman Groves' research on nuclear localization signals and their role in protein trafficking. Learn about experimental questions, methods for measuring nuclear envelope enrichment, and the working model of NLS-mediated INM protein trafficking. Gain insights into the rules for nuclear envelope targeting and the sufficiency of monopartite NLS in enriching artificial membrane proteins at the nuclear envelope.

A Nuclear Localization Signal Targets Tail-Anchored Membrane Proteins to the Inner Nuclear Envelope in Plants

Society for Experimental Biology
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#Conference Talks #SEB (Society for Experimental Biology) #Science #Biology #Plant Biology
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Intro