Explore the role of LINC complexes in nuclear positioning and signaling within Arabidopsis pollen tubes in this 31-minute conference talk from the SEB Annual Conference 2021. Delve into plant LINCs, pollen tube development, and rust sensitivity while examining the position of the nucleus and its impact on tip-based calcium signaling. Analyze example traces, peak counting methods, and nuclear calcium fluctuations to understand the quantitative results presented. Gain insights into the intricate relationship between nuclear positioning and calcium and ROS signaling in plant reproductive processes.